A simple, specific, accurate, precise and reproducible first order derivative spectrophotometric method has been developed and validated for the simultaneous estimation of Sulbactam Sodium (SUL) and Cefixime Trihydrate (CEF) in their combined dosage form. The principle advantage of this method is to get an even order spectrum of narrower spectral bandwidth than its fundamental spectrum, Therefore method shows better resolution of overlapping band. Methanol was selected as a common solvent for Sulbactam Sodium and Cefixime Trihydrate for determination at 255 nm and 275 nm over the Linearity range of 50-300 and 5-30 μg/ml respectively. The method was successfully applied to pharmaceutical dosage form because no interference from the tablet excipients was found. The suitability of this method for quantitative determination of Sulbactam Sodium and Cefixime Trihydrate was proved by validation and recovery studies. The % recoveries of the both the drugs were found to be 99.31% – 99.72 % and 99.24– 99.63 % respectively. Limit of Detection and Quantitation were found to be 2.49μg/ml and 1.01μg/ml for SUL and 7.56μg/ml and 3.06μg/ml for CEF. The method was validated as per International Conference on Harmonization (ICH) guideline.
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